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KMID : 0903520090520050472
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 5 p.472 ~ p.479
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Evaluation of the Antioxidant Activity of the Fruiting Body of Phellinus linteus Using the On-line HPLC-DPPH Method
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Jeon Young-Eun
Lee Yeon-Sil
Lim Soon-Sung
Kim Sun-Ju
Jung Sang-Hoon
Bae Young-Soo
Yi Jae-Seon
Kang Il-Jun
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Abstract
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Phellinus linteus has traditionally been used as a medicinal mushroom in Oriental countries. Bioactive phytochemicals from the fruiting body of P. linteus were investigated using the on-line HPLC-DPPH system. The system was used to separate 10 antioxidants: protocatechuic acid (1) protocatechualdehyde, (2) caffeic acid (3), ellagic acid (4), hispidin (5), davalliallactone (6), interfungins A (7), hypholomine B (8), inoscavin A (9), and methyldavallialactone (10). Of these, compounds 6 and 7 showed the strongest inhibitory effect against the DPPH radical and superoxide anion radical-scavenging capacity. On comparison with quercetin (IC50, 44.0 μM), the IC50 value of compounds 6 and 7 for DPPH radical-scavenging capacity were 19.6 μM and 18.5 μM. Moreover, the antioxidative capacity of compound 6 at 40mM was 2.95 nmol Trolox equivalents per mg dry wt. These results indicated that hispidin dimers such as hispidin with hispidin or hispolon had a stronger effect on radical-scavenging capacity than hispidin itself.
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KEYWORD
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anti-oxidants, DPPH radicals, on-line RP-HPLC-DPPH, Phellinus linteus, superoxide
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